single-cell RNA-seq: a collection

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The motivation to collect resource from the web, is the problem occurring in single-cell RNA seq: cells are lost after cell-death removal kit.

Why happens? how to solve the problem?

  1. The Single-Cell Preparation Guide https://genome.duke.edu/sites/default/files/Single-Cell-Prep-Guide.pdf
  2. Removal of Dead Cells from Single Cell Suspensions for Single Cell RNA Sequencing https://assets.ctfassets.net/an68im79xiti/i1zxeDHukSQKEs2imsKYk/6a8429045a0666f0f4f1a4f908234f0b/CG000093_SamplePrepDemonstratedProtocol_-_DeadCellRemoval_RevA.pdf
  3. Why is my cell recovery low after using the Dead Cell Removal protocol (CG000093)? https://kb.10xgenomics.com/hc/en-us/articles/360044580971-Why-is-my-cell-recovery-low-after-using-the-Dead-Cell-Removal-protocol-CG000093-
  4. An optimized workflow for single-cell transcriptomics and repertoire profiling of purified lymphocytes from clinical samples https://www.nature.com/articles/s41598-020-58939-y
    5.Cell fixation and preservation for droplet-based single-cell transcriptomics https://bmcbiol.biomedcentral.com/articles/10.1186/s12915-017-0383-5
  5. Book_Single Cell Methods and PROTOCOLS http://shaleklab.com/wp-content/uploads/2019/05/2019_Book_SingleCellMethods.pdf?
  6. Droplet-based single cell RNAseq tools: a practical guide† https://pubs.rsc.org/en/content/articlelanding/2019/lc/c8lc01239c#!divAbstract
  7. Complete Guide to Understanding Single-Cell RNA-Seq https://www.activemotif.com/blog-single-cell-rna-seq
  8. What is the best way to separate the viable cells from the dead cells in suspension culture? https://www.researchgate.net/post/What_is_the_best_way_to_separate_the_viable_cells_from_the_dead_cells_in_suspension_culture
  9. A reliable strategy for single-cell RNA sequencing analysis using cryoconserved primary cortical cells https://www.sciencedirect.com/science/article/pii/S0165027020303836
  10. Sampling time-dependent artifacts in single-cell genomics studies https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7212672/
  11. Fresh Frozen Human Peripheral Blood Mononuclear Cells for Single Cell RNA Sequencing: https://assets.ctfassets.net/an68im79xiti/6xwl38JmJc32Y1dgKq6dSt/32c8fc43de6dd213717b34edc44c53c3/CG00039_Demonstrated_Protocol_FreshFrozenHumanPBMCs_RevD.pdf
  12. Single Cell Set-Up: Sample Preparation Tips https://www.biocompare.com/Bench-Tips/345311-Single-Cell-Set-Up-Sample-Preparation-Tips/
  13. Quality Control of RNA-Seq Experiments: https://link.springer.com/protocol/10.1007/978-1-4939-2291-8_8
  14. Best Practices for Illumina Library Preparation https://currentprotocols.onlinelibrary.wiley.com/doi/10.1002/cphg.86

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